Microfluidic Nano-bioreactors Applications
Cell Line Development

Selecting best producer cell

Title: Selecting the best producer cell               

CHO cells are widely used in biopharma production. Clones with high yield are desired. Our collaborator, a renowned research lab, was developing a cell line to produce monoclonal antibodies against TNF-alpha. Our team helped optimize an assay in the droplet format to measure cell expression. Dexter was used to singulate collaborator’s CHO cells. Two separate fluorescence markers (FITC and Cy5 conjugated Anti-human IgG antibody) helped to identify the high producers. Few clones were robotically retrieved and expanded by the collaborator. mAb expression was confirmed by Protein A chromatography. Homogeneous mAb expression observed in enriched cells after single cell sorting and significant yield improvement was confirmed for one of the clones.

Cell line development isn’t restricted to therapeutic applications. Synthetic biology is a big emerging segment with industrial applications too.